FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559666
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1250300.8035519528504018Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1134580.7291801764896496Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG784320.504072516723688Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC772580.49652736761830235TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG647610.4162107335723016Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC646660.41560018062084364Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC627560.4033248528599521Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT555640.3571027809979983Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA534180.3433107111682217Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAG530690.34106773243076044TruSeq Adapter, Index 5 (97% over 37bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC478060.3072430989199897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC413860.26598257314777835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG390460.25094368992239297Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT390210.2507830180930619Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA366980.23585339171162156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG328060.21084000132136513Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTA301570.193815214285448TruSeq Adapter, Index 5 (97% over 38bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC253080.16265130626839935No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC226320.1454529936568047No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC188090.12088305751550195TruSeq Adapter, Index 5 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTATCATCTCGTAT162710.10457165340181467TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)