FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559666
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1299150.8349472283016872Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1201390.772118116160077Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATC820310.5272028332741847Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG798970.513487885922487Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG673390.4327792126129186Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG660550.42452710745847627Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG615210.39538766449099866Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCTC558410.35888302486698626Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA516710.3320829637345686Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG479640.30825854488136184Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG458310.29455002440283745Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA431870.27755737173278655Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA380360.2444525480174189Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG339470.21817306361203384Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCTCGC320770.20615481077807196Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG291760.18751045170249797Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG278660.17909124784555144Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATC197140.12669937773728562Illumina Single End PCR Primer 1 (97% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT177680.11419268254215739No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT170190.10937895453539942No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG161960.10408963791382156No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)