FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20446910
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2649541.2958143797766999Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2516551.2307727671320507Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1550030.7580754255777523Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1499860.7335387107391778Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG1458820.7134672182740571TruSeq Adapter, Index 2 (100% over 35bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1362690.6664527794175257Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1331530.6512133129162304Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1308730.6400624837689411Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1300200.6358907042677843Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1225570.5993913016685651Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1028930.5032202909877336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1000460.48929642669723694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC980410.479490544047976Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA935680.45761437791822823TruSeq Adapter, Index 22 (97% over 37bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT918720.44931972606129733Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC891600.436056108233469No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC879760.4302655022201399Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCTCGTAT817270.39970342707039846TruSeq Adapter, Index 22 (97% over 37bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC718530.3514125117193747No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATT527880.2581710390469758TruSeq Adapter, Index 22 (97% over 37bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG515860.25229240017195753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGCATCGCGTAT514640.25169573299828674TruSeq Adapter, Index 22 (97% over 37bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG459030.22449846945088525No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG377490.18461958310571133TruSeq Adapter, Index 2 (100% over 35bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA350770.17155159385941446No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG266390.13028374458536768No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA261330.12780904302899557No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA248750.12165652413983336No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA240730.11773417108012897TruSeq Adapter, Index 22 (97% over 37bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT221750.10845159488646451No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATTGC219880.10753703126780524TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)