FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7851612
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1096401.3964011466689896Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1041371.326313628335175Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG632190.8051722372424923Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAC608180.7745925295340625Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG565230.7198903868403075Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG540330.6881771539398533Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA536070.6827515165038721Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG534300.6804972023579362Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG534230.6804080486911478Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG438970.5590826444302138Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG403710.5141746688450729Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG401650.5115510037938706Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG383170.48801443576172643Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCT382350.48697006423649053Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA364060.46367548472848635Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC362660.46189241139271786Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT310790.3958295443024948No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT267220.3403377548457565No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCTTA222340.2831775181962634Illumina Single End PCR Primer 1 (96% over 33bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG171930.21897414187048467No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT160280.20413642446926822No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT158620.20202220894257128No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAC154630.19694044993563103Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG110910.14125761690720326No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCT100150.12755342469801106Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACCTTACG92330.1175936864939327Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC82010.10444988876169632No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)