FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28223077
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG10267183.637866983816116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGT5519881.9558037559122272TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGTT4605861.6319482103244802TruSeq Adapter, Index 2 (97% over 36bp)
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC3828091.3563687616343179No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3634101.287634229251474No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGT3031331.0740607765765582TruSeq Adapter, Index 2 (97% over 36bp)
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT3018261.0694298144741625No Hit
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG2935421.040077947560431No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA2785490.9869547533743398No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT2523990.8943000793286996No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG2337550.828240662773942No Hit
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT2334330.8270997524472615No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC2202310.7803224290533594No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC2150630.762011172630114No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGTT2028760.7188301970050962TruSeq Adapter, Index 2 (97% over 36bp)
TTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC1983270.7027121812409044No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT1918810.6798727155086598No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT1863100.6601335495771776No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1712750.6068615410006499Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1599400.5666993715816315Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1599200.5666285075861855Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA1576700.558656308098511No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC1454350.5153052588844229No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1441850.5108762591690481Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC1345010.47656391257409675No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA1270430.45013872867228477No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1224170.4337478865256258Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1210790.4290070852302887Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC1205950.42729217654049556No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC1160520.4111954199749375No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG988480.3502382110922916TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT965850.34221995000757716Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC958810.3397255373678781No Hit
TTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC928400.3289506668603144No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC917760.32518070230258733Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA878620.31131261839380586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTTT856170.30335813490499286TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA854500.3027664205430187No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC854460.30275224774392956No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC829530.29391905071158614Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG786270.27859116849661714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT780550.27656445822686165Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA737090.26116571201644667Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGTACGCCACATGTCCCGCGCCCCGCGGGGCGGGGATTCGGCGCTGG714040.2529986365412956No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT679820.24087380692048568No Hit
GCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG665030.23563341445725425No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA638930.22638566305155178No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGAT630270.22331725204874012TruSeq Adapter, Index 2 (97% over 36bp)
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC622600.22059961782338616No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG618980.2193169795058136No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA610920.21646116048934No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTGT601360.21307386150702134TruSeq Adapter, Index 2 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAA597900.21184791438580564TruSeq Adapter, Index 2 (97% over 36bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG594640.21069283126003588No Hit
CCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCA587070.20801062903240494No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC585920.20760316105859047No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA566650.2007754150973687No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG565890.20050613191467392Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGTACGCCACATGTCCCGCGCCCCGCGGGGCGGGGATTCGGCGCTGGG564030.19984709675702617No Hit
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC557010.1973597705168717No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT550400.19501771546738153No Hit
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG545030.19311501718965654No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC535130.18960724941507973No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG516930.18315862582949405Clontech Universal Primer Mix Short (95% over 21bp)
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT516870.18313736663086028No Hit
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA515420.1826236026638768No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA515190.18254210906911392No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGG503250.17831152854098795TruSeq Adapter, Index 2 (97% over 36bp)
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA473780.1678697188120204No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA465320.16487217180465474No Hit
CCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGC462520.1638800758684108No Hit
GCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCG460950.16332379350415974No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455750.16148132962256384No Hit
CCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCA446980.15837394342225689No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC443970.15730744029079466No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG438780.15546851960897107No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA434360.15390242530961454No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAA431490.1528855269749645No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC423340.14999781916054017No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC418910.14842818166141133No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG416750.14766285051059458No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT407490.14438184752144495No Hit
TTTGAGACAAGCATATGCTACTGGCAGGATCAACCAGGTAGGTAGGTAGA384030.1360695008556296No Hit
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC365570.12952875407596415No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG363820.12890869411581168No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA362610.12847996694336342No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC358900.12716543982784018No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC355360.12591114710844603No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC352000.1247206319849533No Hit
TGCCCCTCGGCGGACTGGAGAGGCCTCGGGATCCCACCTCGGCCGGCGAG347310.1230588712917447No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG346540.12278604490927761No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG343700.12177977617394446TruSeq Adapter, Index 2 (97% over 35bp)
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG342620.12139711059853608No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGG340520.12065303864635313TruSeq Adapter, Index 2 (97% over 36bp)
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG334740.11860506917796383No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA334650.11857318038001313No Hit
CCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCA331290.11738266525652039No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC330690.1171700732701824No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA326420.11565712696741039No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA323710.11469691982911716No Hit
AGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCG319720.11328318311996952No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGA318970.11301744313704705TruSeq Adapter, Index 2 (97% over 36bp)
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT313340.11102262166524224No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGA312390.11068601768687376No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG311550.11038838890600057No Hit
CGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTT302730.10726328670683215No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGAT301680.10689125073074066TruSeq Adapter, Index 2 (97% over 36bp)
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA295930.10485391086166827No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA294630.1043932948912693No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG293360.10394330852018722No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT293120.10385827172565201No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTA291880.10341891495388685No Hit
GTGAGTTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTC289900.10271736139897149No Hit
CTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCC285110.10102016870803987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)