FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28223077
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA4679351.6579871854511115No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG4619491.6367775916141247No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC3549531.2576693887771344No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC3545591.2562733680668483No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG2102870.7450888505176101No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1803220.6389168693406463No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1775200.6289888235786623Illumina Single End PCR Primer 1 (100% over 32bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT1738370.6159392188172821No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC1717790.608647313685889No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1656060.5867751414914824Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1651740.5852444791898488Illumina Single End PCR Primer 1 (96% over 33bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG1505520.533435812119281No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1491520.5284753324380612Illumina Single End PCR Primer 1 (100% over 32bp)
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1345650.47679067735952385No Hit
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG1307070.46312101263799127No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1268210.44935213832283416Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1253470.4441294618584643Illumina Single End PCR Primer 1 (100% over 30bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1172560.41546143250078654No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1078790.3822368482359312No Hit
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC1005810.35637857629768716No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTC1003180.35544671475757234Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA992460.3516484046016669No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA983160.34835322881342806Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC965160.3419754692232884No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG942570.3339713809376632Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG887460.3144448069925189Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT849020.30082474706779844Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC841230.2980645944451769No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC810810.2872861807378409No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG779450.2761747062519087Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA778400.2758026702758172Illumina Single End PCR Primer 1 (100% over 26bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT764180.2707642401996069No Hit
CCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGC759520.2691131091057152No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC739800.26212591915473993No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG725360.25700953868353904Illumina Single End PCR Primer 1 (100% over 24bp)
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG724660.256761514699478No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT722070.25584382595845234No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA714740.2532466605253566No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG701570.24858026642523776No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG694800.2461815201793908No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT691770.24510793064838393No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT690670.24471817867343099No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC676170.23958053900359622No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAA657950.23312482901846598No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA647880.22955682684776008No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA641310.22722894459735912No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA634530.22482665515173983No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT623900.22106023379378512No Hit
GGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTC621470.22019923624911628No Hit
GGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGT612270.21693949245860045No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA604690.2142537470311972No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTC603490.21382856305852121Illumina Single End PCR Primer 1 (96% over 33bp)
AAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGTCCAGCATTA587860.20829054181441664No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT558160.19776723849068617No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA537710.19052139495633308No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG534890.18952221262054453No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG532010.18850177108612218Illumina Single End PCR Primer 1 (100% over 22bp)
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC522280.18505423770767448No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT509650.1805791763952598No Hit
CGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCA507860.17994494363601812No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG488910.17323058006751No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480790.17035350185240256No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG479040.1697334418922501No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT464350.16452848142674167No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC461790.1636214222850329No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA447530.15856881940973339No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA442170.15666966433178067No Hit
ACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTC437240.1549228668440369No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA437170.15489806444563078No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA433320.15353393253329536No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA415300.1471490865436111No Hit
GCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG413690.14657863138027083No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGGGC411000.14562551064152218No Hit
GCGGGGGCGGCGGCGCGCGCGCGCGTGTGGTGTGCGTCGGAGGGCGGCGG404610.1433614059870226No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA393570.13944971343840362No Hit
CGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCT390360.13831234631149536No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG388090.1375080399631833No Hit
GGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATT382570.1355521936888738No Hit
CCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGG378720.1341880617765384No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA374690.13276015226830157No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA362690.1285083125415418No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT355290.12588634471003995No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTC347060.1229702912974372Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAG345800.12252384812612743No Hit
GTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCA334330.11845979798729954No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG331610.117496047649234No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC328610.11643308771754404No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTCTC320700.1136304166976549Illumina Single End PCR Primer 1 (96% over 33bp)
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC318580.11287925834592735No Hit
GGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCT317880.11263123436186634No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT314990.11160724962767171No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA314250.11134505284452154No Hit
CGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGCTGG313290.11100490566638074No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT311720.11044862330212968No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGGGCGCGGGACATGTGGC289470.10256500380876259No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTCTCGGTGTAGATC289410.1025437446101288Illumina Single End PCR Primer 1 (96% over 33bp)
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC289030.10240910301878141No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA286110.10137448868526985No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)