FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140797
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG1510673.6482590187347994No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGTT1005212.427576140535264TruSeq Adapter, Index 2 (97% over 36bp)
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC553651.337061440104405No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA544411.3147468953440604No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGTT508661.2284108590689184TruSeq Adapter, Index 2 (97% over 36bp)
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT433771.047551956785131No Hit
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG425411.027362606763867No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA399770.9654421600479327No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA391830.9462671075157754No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGGT389210.9399398231789677TruSeq Adapter, Index 2 (97% over 36bp)
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT363660.878236725924985No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTTT357630.8636743119742407TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGGT343660.829936845491339TruSeq Adapter, Index 2 (97% over 36bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT331480.8005222183072486No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG326060.7874329507097304No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC313280.7565693271126307No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT308640.7453637548520248No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC306090.739205520096735No Hit
TTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC289900.7001067668856986No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT275810.666079501120195No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC273360.6601627657670733Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG254200.6138914803116404Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC249510.6025651583499505Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC226640.5473342450740764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA226520.5470444457914745No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC224740.5427457564328799No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC214850.518861465558442No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC194060.468653739847667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG190310.45959751226635837Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC171920.4151857722076209No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGGAT168480.4068781927730337TruSeq Adapter, Index 2 (97% over 36bp)
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC168130.406032944865445No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG156520.3779948642737135TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT150300.3629736014588496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC144150.3481213882255035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC140780.33998285837243414No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA135480.3271833900575179Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC131260.3169921152860186No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC130750.31576046833496063Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA126650.3058589928460632No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC122820.29660956574302No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG120480.29095847973228345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT119930.2896302330203581Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113340.2737154224174718No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA111300.2687888346132399Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGTTT107060.25854925996130695TruSeq Adapter, Index 2 (97% over 36bp)
TTCCGTACGCCACATGTCCCGCGCCCCGCGGGGCGGGGATTCGGCGCTGG105950.2558686165972396No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTAT98710.23838405988025976TruSeq Adapter, Index 2 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAA96880.23396462082058114TruSeq Adapter, Index 2 (97% over 36bp)
CCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGCCC95270.23007648044567267No Hit
GCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG94960.22932783229895115No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCTCGTGT94920.22923123253808386TruSeq Adapter, Index 2 (97% over 36bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT93550.22592269072837912No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA90030.21742191177205739No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC87500.21131197689720121No Hit
CTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGG87230.21065992851134696No Hit
TCCGTACGCCACATGTCCCGCGCCCCGCGGGGCGGGGATTCGGCGCTGGG85960.20759288610381044No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA84280.20353569614738418No Hit
CCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACCA84130.20317344704413187No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG83960.20276289806044584No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG83930.2026904482397954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCAC81740.19740161133231116No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT79920.19300632221284936No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC79210.19129167645745493No Hit
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG78140.18870763285425488No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGGAT76350.18438479355544357TruSeq Adapter, Index 2 (97% over 36bp)
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT75410.1821146991750622No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA74440.17977215497403035No Hit
GCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCA73030.17636701340345834No Hit
CCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGTCCGC72720.17561836525673682No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG72690.17554591543608633Clontech Universal Primer Mix Short (95% over 21bp)
CTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGA67090.16202194891466545No Hit
CGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCA64970.156902161588699No Hit
CCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCA64870.15666066218653077No Hit
GCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCG64600.15600861380067654No Hit
GTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCC63820.15412491846376436No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTG63620.1536419196594279No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA62340.15055072731167454No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAA61590.14873948179541283No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC60530.14617958813242957No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59990.14487549136072114No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG59790.14439249255638467No Hit
TTTGAGACAAGCATATGCTACTGGCAGGATCAACCAGGTAGGTAGGTAGA57640.13920025540976771No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC57540.1389587560075995No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT56290.1359400134804966No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG56220.13577096389897889TruSeq Adapter, Index 2 (97% over 35bp)
TGCCCCTCGGCGGACTGGAGAGGCCTCGGGATCCCACCTCGGCCGGCGAG54460.13152057442081802No Hit
AGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCC52460.12669058637745342No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG52440.12664228649701978No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGA51470.12429974229598793TruSeq Adapter, Index 2 (97% over 36bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC51190.1236235439699169No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA51160.12355109414926645No Hit
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC51070.12333374468731502No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC50410.12173984863300472No Hit
CCTGCGGCGGCCTCCACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCG49780.12021840239934485No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG49230.11889015568741959No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAAGAGAATCGCGTGT48870.11802075783961398TruSeq Adapter, Index 2 (97% over 36bp)
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA48790.11782755831787937No Hit
CCTGCAATTCACATTAATTCTCGCAGCTAGCTGCGTTCTTCATCGACGCA47940.11577481339944942No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC47780.11538841435598027No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG47480.11466391614947558No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA46890.11323906967668303No Hit
AGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCG46620.1125870212908288No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA46110.11135537433977082No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT45820.11065502607348296Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTT45650.11024447708979696No Hit
GGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGA45020.10872303085613712No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG43540.10514883970404731No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTA43270.10449679131819309No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG43060.10398964257363982No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA42280.10210594723672761No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT41970.10135729909000611No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41620.10051205118241731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)