FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4140797
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA666641.6099316146142881No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG653461.5781019934085154No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG520951.2580911355953939No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC507421.2254162664820323No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC502961.2146453931453294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG453981.0963589859633303No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG274450.6627951092507071Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG254060.6135533811486049Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT248310.5996671655239317Illumina Single End PCR Primer 1 (96% over 33bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT243790.5887513925459278No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC238340.5755896751277592No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG226870.5478896936990633Illumina Single End PCR Primer 1 (100% over 32bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG212030.512051182417298No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT194230.469064288831353No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG193980.46846054032593243Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG190690.4605152099945976Illumina Single End PCR Primer 1 (100% over 30bp)
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG184180.44479359891344594No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC166780.4027727029361739No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG163210.3941511742787681No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTC152780.3689627866326217Illumina Single End PCR Primer 1 (96% over 33bp)
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC149000.3598341092306626No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA146860.35466602202426245Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG143750.34715539061683054Illumina Single End PCR Primer 1 (100% over 28bp)
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC143320.34611694318750713No Hit
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC140900.34027265765503595No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG129890.31368357347631387Illumina Single End PCR Primer 1 (100% over 24bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA129710.313248874552411No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT129080.31172742831875117Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC121360.29308367447136385No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA114980.27767601261303076Illumina Single End PCR Primer 1 (100% over 26bp)
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC114920.27753111297172983No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG110290.2663496906513408Illumina Single End PCR Primer 1 (100% over 22bp)
CCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGC109010.26325849830358744No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT104740.252946473831004No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG102520.24758518710286934Illumina Single End PCR Primer 1 (100% over 24bp)
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG102190.24678823907571418No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA101890.24606374086920949No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA101410.244904543738802No Hit
GGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACC98140.23700751328790087No Hit
AAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGT97650.23582416621727656No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG97220.23478571878795315No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97020.23430271998361668No Hit
GGGAAGTCGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAA96950.23413367040209893No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG95820.2314047271575979No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTC93770.22645398941314923Illumina Single End PCR Primer 1 (96% over 33bp)
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT92910.22437709455450242No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT91220.22029575465785933No Hit
GGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCA90830.21935390698940324No Hit
GGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTC89210.2154416166742779No Hit
GGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGT88940.2147895682884237No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA87870.21220552468522363No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA87780.21198817522327223No Hit
AAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGGTGTCCAGCATTA80870.19530056653344754No Hit
CGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCT80420.19421381922369052No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA80360.19406891958238956No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC75440.18218714899571267No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA74970.181052101805522No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG74020.17875785748492382No Hit
CGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCA72970.17622211376215738No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG70780.17093327685467316Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT68360.165088991322202No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG67220.16233589813748414No Hit
GGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGG66610.16086275178425796No Hit
GCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGC65140.15731271057238497No Hit
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA63280.1528208216920559No Hit
GCGGGGGCGGCGGCGCGCGCGCGCGTGTGGTGTGCGTCGGAGGGCGGCGG61640.14886023149649694No Hit
CGGCGAGTGAACAGGGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGGGC61390.14825648299107635No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT61160.14770103436608942No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA60920.14712143580088566No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA60850.14695238621936793No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA60490.14608298837156228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60460.1460105385509118No Hit
ACGGGCTTGGCGGAATCAGCGGGGAAAGAAGACCCTGTTGAGCTTGACTC60120.14518944058353983No Hit
GCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTTG57680.139296855170635No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA56290.1359400134804966No Hit
GGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATT55010.1328488211327433No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCG54630.13193112340450402No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA54430.13144812460016755No Hit
CGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCT54210.13091682591539744No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTC53710.1297093289045563Illumina Single End PCR Primer 1 (96% over 33bp)
CCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGACTATCAGG51590.12458954157858983No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA50490.12193304815473929No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTCTC50440.12181229845365518Illumina Single End PCR Primer 1 (96% over 33bp)
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT50210.12125684982866826No Hit
GGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAG49510.11956635401349064No Hit
GTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCA49200.11881770586676912No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAGC47760.11534011447554661No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG47550.11483296573099333No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA47140.11384281818210358No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG46210.11159687374193905No Hit
GGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCT46040.11118632475825307No Hit
GGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCT45510.10990637792676143No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTCTCTCGGTGTAGATC45380.10959242870394274Illumina Single End PCR Primer 1 (96% over 33bp)
CGGCAAGCCGGATCCGTAACTTCGGGATAAGGATTGGCTCTAAGGGCTGG45160.10906113001917263No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC44490.1074430840246455No Hit
GGAAGAGCCCAGCGCCGAATCCCCGCCCCGCGGGGCGCGGGACATGTGGC43780.10572843826925106No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT42980.10379644305190522Illumina Single End PCR Primer 1 (100% over 31bp)
GGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCT41880.10113994962805468No Hit
GGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGA41820.10099504998675377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)