FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23944662
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2445921.0214886307436706Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2120790.8857047136434834Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1486770.6209191844094522Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1285460.5368461663814674Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1276310.5330248553936573Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1275730.5327826302162879Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT989770.41335726518085747TruSeq Adapter, Index 6 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG972260.40604457060199894Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG966200.40351373512810496Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC961170.40141305815884976TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC900640.37613393749304125Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA800350.3342498632889451Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC758880.31693076310703405Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT724430.3025434228305248Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG722060.3015536406402396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC636580.2658546610513859No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTAT589750.24629706612688873TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC536670.22412928610142835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAA503140.21012616507178092TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC466130.19466969297791714No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA319530.13344519124972404No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG310220.12955705952332924No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATT267190.11158645714021771TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG258640.10801572392210004No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)