FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23944662
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2408891.0060238060574838Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT2095590.8751804473163998Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1442540.6024474264869556Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1222550.510573087229212Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1163200.48578676951046545Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1134410.47376321286138845Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG949600.39658108349994664Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGT916750.382861950609284Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG891460.3723000976167465Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG804470.3359704973075001Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG754270.3150054905765636Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG734670.30681995009994295Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA688000.28732917591403045Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG648200.2707075171910967Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT527650.22036226696371827Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT495960.20712758442779441No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT400110.16709778571942258No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTC259030.10817859947240016Illumina Single End PCR Primer 1 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)