FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6199952
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC724291.1682187216933293Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC622751.0044432602058855Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG431600.6961344216858454Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC373480.602391760452339Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT365490.5895045639063012Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA363170.5857625994523828Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCTCGTAT311130.5018264657532833TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG284060.4581648374051928Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC283430.45714870050606843TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG274070.44205180943336336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC264100.42597103977579187Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA230790.3722448173792313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC225690.3640189472434625Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT214600.34613171198744763Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG205270.3310832083861294Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATTCCATCGCGTAT179970.29027644084986465TruSeq Adapter, Index 6 (97% over 37bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC172000.2774215026180848No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC161580.2606149208897101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAA148430.2394050792651298TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC130460.2104209839043915No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA98480.1588399394059825No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG83760.1350978201121557No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT80950.13056552695891838No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAATT78600.12677517503361316TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG69390.11192022131784247No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG62660.10106529857005346No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)