FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6199952
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG684801.1045246801910724Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT589450.950733166966454Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG401930.6482792124842257Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG334000.5387138481072111Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA310720.5011651703109959Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG286000.4612938938882107Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG266110.42921300035871246Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGT260040.41942260198143466Illumina Single End PCR Primer 1 (97% over 35bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG249870.40301924918128396Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG206770.3335025819554732Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG196290.31659922528432477Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA191000.3080669011631058Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG187700.3027442793105495Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG160360.2586471637199772Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT151400.24419543893243045Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT110050.17750137420418738No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT96950.15637217836525186No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTC91030.14682371734490848Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72550.11701703497059332No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG66450.10717824912192869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)