FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005408274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31184352
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG4621681.482050997885093No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGT3167261.0156568268598303TruSeq Adapter, Index 16 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA2285500.7328996286342586No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC1988640.6377044486927289No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1862660.5973059821797805Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1554740.4985641516617052Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG1461400.468632473107025No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGTT1353290.43396444473176804TruSeq Adapter, Index 16 (97% over 37bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1309850.4200343813461315No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA1262380.4048120031482456No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT1192550.38241936212110483No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT1137880.3648881336383068No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT1035250.3319773968687885No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT995070.3190927295843761No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC935670.30004471473385114No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG917980.2943719978532823Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCACACCCCCGCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACAC882570.2830169438826242No Hit
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC881310.2826128950827646Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC855670.27439082267927195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAGAATCTCGGGG845840.27123860069306555TruSeq Adapter, Index 16 (97% over 37bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT803490.2576580715866727No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG789700.2532359819437646Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA760300.24380817661370677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC725290.23258139210332157Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT713910.2289321259585577Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC701220.22486277733140006TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC696280.2232786494970298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA693400.22235510938306494Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA672080.21551834715051957No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG667890.21417472455416103Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGAC667890.21417472455416103No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT640730.20546522820163138Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC617540.19802880624231026No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC590010.18920066063902818Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG563490.1806963954229352Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTA560100.1796093117471224No Hit
TCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTC518780.16635907650093226No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC456460.1463746945904151No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC447700.14356559341043867No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC447620.1435399395183841No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA400210.12833680173953912No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC390710.12529040205805783No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGT382500.12265767138595665TruSeq Adapter, Index 16 (97% over 37bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT367770.11793414851140727No Hit
TGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTC367080.1177128836924365No Hit
TTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGC357670.11469534463951662No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC357660.1146921379030098No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG334610.1073006102547842No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA332040.10647647897253083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)