FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31184352
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA2110530.6767913599743871No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG2029610.6508424481611803No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1712750.5492337952060059Illumina Single End PCR Primer 1 (100% over 32bp)
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC1450830.4652429526193137No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1432460.45935217765628095Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC1430500.4587236573009437No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1070470.343271522845817No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC996570.31957374006039957No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG922000.2956611059290249No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG832650.2670089152405668Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG813140.2607525723157563Illumina Single End PCR Primer 1 (100% over 32bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG802630.257382292247086No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG710350.2277905277621289Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTA651840.20902791246071106Illumina Single End PCR Primer 1 (96% over 33bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT650670.20865272428941284No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG638630.20479181353519868No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG632820.2029286996247349Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG621000.1991383370736708Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG613890.19685834741732006Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG593470.19031019147038875Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA593000.19015947485456808Illumina Single End PCR Primer 1 (100% over 26bp)
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG585150.1876421866967125No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA556470.17844526639514585Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG542320.1739077342379922Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC537620.17240056807978565No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG533040.170931882759661Illumina Single End PCR Primer 1 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG511450.16400853864143145No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT496160.1591054385225No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA467360.14987003738285148No Hit
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC451760.14486752843220857No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT423190.13570588223221697No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG406500.13035383900233039Illumina Single End PCR Primer 1 (100% over 22bp)
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT401020.12859654739659174No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA400000.12826946027289585No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC367150.11773533084798427No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA366110.11740183025127475No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG355860.11411492533178179No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATG353810.11345754434788319Illumina Single End PCR Primer 1 (96% over 33bp)
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA353250.11327796710350115No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT352990.11319459195432377No Hit
CCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGC348820.11185738283097882No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC344810.11057148149174305No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT343060.11001030260304911No Hit
GGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGT334060.10712423974690896No Hit
GGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTC320890.10290096776742388No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG317630.10185557166619977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)