FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077737
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA208710.6781281181595439No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG200790.652394925232403No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG178870.5811737650098108Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG151950.4937069021817004Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC147840.4803529346399643No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC142570.4632299640937481No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG135600.44058345466165566No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC116080.3771602316897123No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC100130.32533644037810894No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG87230.28342252765587184Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG86920.2824152940943297Illumina Single End PCR Primer 1 (100% over 30bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG80200.26058106979251316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74030.24053387277730356No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG73040.23731722366141095Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTA69490.22578277481149298Illumina Single End PCR Primer 1 (96% over 33bp)
TGCTGTTCACATGGAACCCTTCTCCACTTCGGCCTTCAAAGTTCTCGTTT65530.2129161783479225No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG65330.21226635024370177Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG64800.21054430576751682Illumina Single End PCR Primer 1 (100% over 28bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG63710.20700274259951387No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA60970.19810009757168984No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA59530.1934213352213006Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG58550.190237177510619Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG57310.18620824326445048Illumina Single End PCR Primer 1 (100% over 24bp)
CGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGG57260.1860457862383953No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG56890.18484360424558693Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA56350.18308906836419095Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTC54850.17821535758253548No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT49920.16219709481349445No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG49360.1603775761216764Illumina Single End PCR Primer 1 (100% over 22bp)
GCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCC46040.14959042959161228No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA43340.1408177501846324No Hit
CCGCGGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTT42000.13646390188635352No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT40070.13019306068062345No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTATG38700.12574173816671144Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC38420.12483197882080244No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT36280.1178788181056406No Hit
CCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGC36150.11745642983789714No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA35440.11514954006791353No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG34830.11316756435004031No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA34600.11242026203018647No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG34500.11209534797807609Illumina Single End PCR Primer 1 (100% over 22bp)
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC34480.11203036516765402No Hit
GGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGT33180.10780648249021926No Hit
GGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTC32920.10696170595473231No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG32200.10462232477953769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)