FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005408300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34516721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1638020.47455840315770437Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1498000.43399255682485016Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1120150.324523873516259Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTAT1119500.3243355589889318TruSeq Adapter, Index 6 (97% over 36bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1104040.31985657038511855Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG1084620.31423031173789656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1069860.30995412339428186Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT993600.28786048361893934Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC964750.27950221575218576Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG949100.27496818136346146Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC932570.27017919807620194TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG901850.2612791638000609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA886920.2569537239646837Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC866530.25104644209975796Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT816990.2366939779708507Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC726920.21059937877644866No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC696340.20173990455234725Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC673250.19505039311237007No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCGCGTAT651820.18884180800372086TruSeq Adapter, Index 6 (97% over 36bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT622070.18022279694528343TruSeq Adapter, Index 6 (97% over 36bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG440660.12766566094154772No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG439020.12719052890336832No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA366630.10621808485226623No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCA348640.10100611816516405TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)