FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34516721
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1656100.47979644416397493Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1551360.4494517309451266Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1114510.32288988284837367Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1109310.3213833666297561Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1072510.310721867236462Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG980920.2841869017627717Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG920340.2666359878158762Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG911040.2639416415018101Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA884780.256333734597791Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG829230.24024008537775068Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG806550.23366935694731839Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG758390.21971669904565966Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA750150.21732945026846553Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG733050.21237532962647293Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA726170.21038209278337883Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTG656710.19025851267853627Illumina Single End PCR Primer 1 (97% over 37bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT533970.15469893562601153No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT521430.1510659138218836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG376890.10919055723746182No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTA375290.10872701378557947Illumina Single End PCR Primer 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)