FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10687588
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC570020.5333476552426983Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTAT554560.5188822772734129TruSeq Adapter, Index 6 (97% over 36bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC520930.48741586969857Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG396690.3711688736504438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC385040.3602683786089059Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG378560.3542052706373038Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA360930.3377095000293799Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT334670.31313894210742405Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC330520.30925593314412947Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC330430.3091717233111905TruSeq Adapter, Index 5 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG319990.2994033826902759Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG311240.291216315598992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC303500.283974269966245Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA302660.28318831152548174Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT286470.2680398982445805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCGCGTAT278360.26045165663197345TruSeq Adapter, Index 6 (97% over 36bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC245120.22935015833319924Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC242010.22644024077275435No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC225880.21134796738047912No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT220790.20658543349537803TruSeq Adapter, Index 6 (97% over 36bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG143380.13415562051980295No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG141210.1321252278811646No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCA125020.1169768146002634TruSeq Adapter, Index 6 (97% over 36bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA110660.1035406679224536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)