FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10687588
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG547070.5118741478432739Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA511550.4786393337767137Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG370130.3463176162853583Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG368670.344951545662127Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG351340.3287364744973328Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGG329300.30811442207540185Illumina Single End PCR Primer 1 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG290820.27211004016996165Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG273390.25580140252412426Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA269520.2521803797077507Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG269240.2519183935608296Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG247480.2315583272858198Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA241130.2256168557395738Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA241110.22559814244336515Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG224570.2101222464788126Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTG221640.20738074858424557Illumina Single End PCR Primer 1 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG201140.1881996199703806Illumina Single End PCR Primer 1 (100% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG186470.17447341720133672No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT151040.14132281296771546No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT137070.12825157556597427No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTA125300.11723880074718449Illumina Single End PCR Primer 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)