FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43817663
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT617540.14093403383927616Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG610120.1392406527933724Illumina Single End PCR Primer 1 (100% over 32bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG532770.12158795415446963No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC477870.10905876016253993No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)