FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43883470
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1289590.2938669161759542Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1189090.2709653543805902Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT877940.2000616633096699No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA727260.16572527195319786TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG723660.16490491750082661Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG637280.14522096816865213No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG627770.1430538651569714Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC611960.13945114185364102Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC576000.13125671237939934Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA546310.12449106690970427No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT517870.11801026673597143Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA517680.11796697025098517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC510080.11623511085153476No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG484210.11033995260630028No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG483180.11010524008242739No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC474030.10802017251598381No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA470540.10722488444965723No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG466450.1062928706412688No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC460340.10490054683460538Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA456800.10409386495644032No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACG447090.10188118669740565TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)