FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43883470
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1315430.29975523813408556Illumina Single End PCR Primer 1 (100% over 32bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1267220.28876932475941397No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT1200910.27365885149920915Illumina Single End PCR Primer 1 (96% over 33bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1112020.25340293281274245No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG734820.1674480163031775Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCG726730.16560449754770987Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG637380.1452437557923291Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG624990.1424203692187514Illumina Single End PCR Primer 1 (100% over 32bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT621070.1415270943706138No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA584780.13325746573823813No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC576420.13135242039884265No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG571590.13025177817524458Illumina Single End PCR Primer 1 (100% over 28bp)
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA559360.12746485179955003No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT542240.12356361062605122No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC517740.11798064282519136No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT504120.11487696848038681No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA496130.11305623734859618Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG489200.11147705502778152Illumina Single End PCR Primer 1 (100% over 24bp)
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA471540.10745276068642702No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG464540.1058576270290385Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCGCA449750.10248733748721331Illumina Single End PCR Primer 1 (96% over 33bp)
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG442200.10076687189960137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)