FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43460478
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1020800.23488006735682934Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC942410.21684299008400232Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT687110.1580999638338078No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG648590.14923673872155754No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT589980.13575092294198882TruSeq Adapter, Index 22 (97% over 38bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA553310.12731337193300085No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA529140.12175199729740661No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT527880.12146207871896852TruSeq Adapter, Index 1 (97% over 35bp)
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC498320.11466049683116691No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC463220.1065841935746772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)