FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43460478
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG1687750.38834133393562764No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1379820.31748845468289605No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG944580.21734229430242347Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC866350.19934203208717585Illumina Single End PCR Primer 1 (96% over 33bp)
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA592750.13638828362633287No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA582370.13399990676586668No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT535140.12313256195663565No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG531980.12240546456944168No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT483240.11119067765430468Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC446130.10265188523697324No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)