FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15553913
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG506080.32537149976343577Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT447770.2878825411971894Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGA231250.14867641345300053Illumina Single End PCR Primer 1 (97% over 36bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG215530.13856963196335223Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG172020.11059596385809796Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG163680.10523396909832272Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG161420.10378095852792799Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG158750.10206434869476254Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA157520.10127355090645035Illumina Single End PCR Primer 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)