FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38498091
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT7004081.8193317689440758TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCGCGTAT3477310.9032421893334919TruSeq Adapter, Index 23 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3322700.8630817564434559No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG2311820.6005025028383875No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT1699480.4414452654288754No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1650020.4285978751517835No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC755970.1963655808284104No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC731560.1900250066944878Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA659930.17141888931583646No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA597930.15531419467006818No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT597170.15511678228408782No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT559920.14544097783965443No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC556510.1445552196341372Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG523550.13599375615793521Clontech Universal Primer Mix Short (95% over 21bp)
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC521690.13551061531856215No Hit
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG512260.1330611432135687No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC506600.1315909404442937No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC505160.13121689592348879No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT476310.12372301785041756No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTGGTAT473020.12286843002163406TruSeq Adapter, Index 23 (97% over 37bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC401120.10419217929533181No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA400910.10413763113604776No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT398140.10341811493977714No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA397990.10337915196885997Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG389760.10124138363120395Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)