FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38498091
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1574670.40902547609438605No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC1443730.37501340001508127No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG1088760.2828088281052689No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA1078370.2801099929864055No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG929900.2415444443725794No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC796520.20689857063302175No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC761340.19776045518724553No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG760650.19758122552102647No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG759190.19720198593743257Illumina Single End PCR Primer 1 (100% over 32bp)
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA617140.16030405247886187No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT582680.15135295929348808Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA562280.14605399524875143No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT512500.1331234839670362No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC498850.12957785361357269No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG481420.12505035639299622No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA454370.11802403397093118No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG409900.10647281185968416No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG403470.10480259917303432Illumina Single End PCR Primer 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)