FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266626
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCTCGTAT1127722.643118942227418TruSeq Adapter, Index 23 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACACAGATCGCGTAT465771.0916588423733415TruSeq Adapter, Index 23 (97% over 37bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA415400.9736030296538764No Hit
TAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAG264950.6209824812392744No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT227870.5340754029061839No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG180050.42199620965137324No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA123570.2896199479401288No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC90190.21138482726163485Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC85700.20086128945916515No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCA74080.17362665487905432No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC68520.1605952806737689Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT66810.15658742997394193No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA62790.1471654651708399No Hit
CATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTT62330.14608732989486306No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC59950.14050915172785242No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG57130.1338997137316465Clontech Universal Primer Mix Short (95% over 21bp)
CCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGATATGGGTACGGCCCGG56810.13314970658314088No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCAC56620.13270438983871566No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC56450.13230594854107205No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT53020.12426680941802727No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG49460.11592297989090208Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA47540.11142293699986827Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC46320.10856353474619056No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT45560.10678226776848966No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG45030.1055400684287772Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT44990.105446317535214Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC42850.10043064472958257Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)