FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38076688
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC1065930.27994294041540585No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG896560.23546165569862587Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG882240.23170082439943307Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG873870.2295026290101702Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG860600.2260175569892003Illumina Single End PCR Primer 1 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG632640.1661489045475804No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC622190.1634044431595521Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG593330.15582500242668162Illumina Single End PCR Primer 1 (96% over 33bp)
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG592640.15564378918670657No Hit
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG577770.15173851255130172Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG569510.1495692062292813Illumina Single End PCR Primer 1 (100% over 30bp)
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC534060.14025904774070685No Hit
GCCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGT530730.13938449688691412No Hit
CTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCG521140.13686589547914463No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG510150.13397961503374453No Hit
CGAGAATCACGAGCGACGGTGGTGGTGGCGTGTCGGGTTCGTGGCTGCGG492990.12947292054392964No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG462570.1214837803119851Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA454390.11933548422068642Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG431450.11331080056122529Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG428260.1124730176111956Illumina Single End PCR Primer 1 (100% over 28bp)
CCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCC427650.11231281460194226No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCGG419680.11021967036628816Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG414630.10889339955197784Illumina Single End PCR Primer 1 (100% over 24bp)
CCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTG408270.1072230862095989No Hit
GGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGG399440.10490408199368602No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT394080.10349639653532891No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA386080.10139537346315415Illumina Single End PCR Primer 1 (100% over 26bp)
GTTCGAGGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGG383460.10070728840701693No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)