FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8337531
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT641430.7693284738611467TruSeq Adapter, Index 22 (97% over 37bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC241700.2898939746071109Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC241080.289150349186108Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCGCGTAT237780.2851923429130278TruSeq Adapter, Index 22 (97% over 37bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG237240.2845446691592511Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC236430.283573158528586Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG170340.20430508744135406TruSeq Adapter, Index 2 (100% over 35bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC161810.1940742409233621Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC160760.1928148752910184Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG158640.19027215610952453Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA136220.1633817013693862Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG132700.15915982801143408Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT128530.1541583473572692Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG127590.1530309152673615No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA124710.1495766552472189Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC118190.1417565943682848Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT115030.1379665035128505Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAA114030.1367671076725232TruSeq Adapter, Index 22 (97% over 37bp)
GGCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATCCCC114020.1367551137141199No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG112760.1352438749553075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA112280.13466816495195041No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA112140.13450024953430456No Hit
CAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCCT107780.1292708836704775No Hit
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGG105910.12702801344906545No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG97240.11662925151342765No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC97100.11646133609578184No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC93820.11252731773950825No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC93340.11195160773615113No Hit
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC92520.11096810314708275No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG91350.10956481001389981No Hit
GGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC88340.1059546285345146No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG87400.10482719644460692No Hit
GCACGCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGG85100.10206858601185412No Hit
GGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCG83440.10007758891691076No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)