FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8337531
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG226650.2718430672101849Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG225620.27060768949464775Illumina Single End PCR Primer 1 (100% over 32bp)
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC225390.2703318284513725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223520.26808895822996043No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG219570.2633513446606675Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG218540.2621159669451304Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTC160650.19268294174858241Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG150960.18106079605581077Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG144180.1729288922583916Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG142700.1711537864147072Illumina Single End PCR Primer 1 (100% over 30bp)
GCCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGT121150.14530680605565363No Hit
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG119930.14384354313045433No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC111970.13429635224144895No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG109940.1318615786855845Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA109310.1311059593061783Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCGG108540.13018242450912626Illumina Single End PCR Primer 1 (96% over 33bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG108400.13001450909148046No Hit
CTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCG107450.1288750830431695No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG102420.12284212196632313Illumina Single End PCR Primer 1 (100% over 28bp)
CGAGAATCACGAGCGACGGTGGTGGTGGCGTGTCGGGTTCGTGGCTGCGG100670.12074317924575034No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG96170.11534589796427742Illumina Single End PCR Primer 1 (100% over 22bp)
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG94660.11353481024538319No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA93090.11165175877606932Illumina Single End PCR Primer 1 (100% over 26bp)
CCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTG91720.1100085864748209No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG91180.10936091272104416Illumina Single End PCR Primer 1 (100% over 24bp)
GGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGG90850.10896511209373613No Hit
CCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCC89830.1077417283366023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)