FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005408356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28424264
Sequences flagged as poor quality0
Sequence length151
%GC70

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCTCGTAT985890.346848030963968TruSeq Adapter, Index 7 (97% over 37bp)
CCCAGACCCGCCGCGACGCCGCCGAGAACCGCCCCCGCGCCCGCCGACAC856360.3012778096910443No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA834070.29343591798894075No Hit
GGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCG713950.2511762485741056No Hit
GGCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATCCCC699960.24625439729943402No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA695620.2447275327867768No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACAGACTATCGCGTAT677420.23832455257240784TruSeq Adapter, Index 7 (97% over 37bp)
GCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGC674990.2374696491701597No Hit
CGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCCCGCC644460.2267288257666056No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG633320.2228096389760523No Hit
GGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGCG610510.21478480498210964No Hit
GCACGCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGG586440.20631668774255685No Hit
CGGGCGGCGAACCGCGGCGACCGGGACGCGCTCCCCCGACCCTCTCTCCC584160.2055145561552623No Hit
GGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCGC573030.20159888748570587No Hit
GCGGAGGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGG563690.19831296247459565No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT535240.18830390823839802No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG525130.18474708791052602No Hit
CGGGACCTTCCACCCGGCCGGGGCCGACGAACCCCGAACCCCGAGCCGCG498940.17553312901962914No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC486650.17120935831443165Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC486100.1710158616596018Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA484770.1705479515670133No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG436820.1536785613868489No Hit
GGGAGAGCGAGCGGGGCCGTGCCCGGCGGCGCGGAGCGGCGCGGCGGAGG436260.1534815466110222No Hit
CGAGGAAACACCTGCGCGCGGCCACCTCGAGCGTTCGCGTTCAGGGCGGG429610.1511419961480797No Hit
GGCGACGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGG386570.13600000337739615No Hit
GCGGGAACGACACACCACCGTTCGGCCTCGGGCACCTGAGGGACAACCCG372940.13120480445861324No Hit
CCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACACGCCCCCACCG366040.1287773009707481No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG358720.12620203640101288Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC353700.12443593966056607Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGGCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATC351620.12370417049320961No Hit
GGCAAGGGCACCCGGGAGCCCGCAGAGGCGGCGGCTCGGGGAGAAACCTC348410.12257485365320275No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA347690.12232154894142554TruSeq Adapter, Index 7 (100% over 35bp)
CGAGAGACCACGGGCGAGGCCGGGGCGACGGGGAAGGCGCGAGAAAGGCG345870.12168125091998863No Hit
GCGGCACCGCGCGGCACCCGGGGCGGCCGACCGGCCCTCGGCGATCCCCG327220.11511995526075891No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG321070.11295631084766171Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCGCCTTCCTCCTCCCCCACCACCACACCGCACGCAACACGCCCCCACC315200.11089117382247786No Hit
CACGCAACACGCCCCCACCGCCGACGACGCGCGACGACGACGACGACGGG314350.11059213353774085No Hit
CGGGGAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGG311580.10961761402159789No Hit
CCGGAACCGTCTCCTCCCCCGCACGCGCCGCAGGCCGACCCCCGGAACCC305880.10761228505336146No Hit
CCGGCCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAG299280.10529032519540346No Hit
GCCAGGCGGGCCCGACCCCGTGCCACGCAAACGCGGTCGTCGGCACCGGT295500.103960475458573No Hit
GAGAAACCTCAGGCACGGCCGGGCCACCAGGAAAACACGGCCGCGGGATC290410.10216975187114782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)