FastQCFastQC Report
Wed 11 May 2022
EGAF00005408357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28424264
Sequences flagged as poor quality0
Sequence length151
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC1535040.5400456455090623No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1155690.40658572549143224No Hit
GGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGG935340.32906392932460804No Hit
CTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCG748240.2632398854724963No Hit
CCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCC695450.24466772472982942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA565200.19884419874512843No Hit
GGGGGACCGGCCGCGACTGCGGCGGCGGTGGTGGGGGGAGCCGCGGGGAT526540.1852431429710898No Hit
CGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGTCCT524870.18465561676460646No Hit
GGGAAAGAGAGAGCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGG521530.18348056435163984No Hit
GAAAGAAGGGCGTGTCGTTGGTGTGCGCGTGTCGTGGGGCCGGCGGGCGG517340.18200647165393624No Hit
CCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGGGGCGTGT517010.18189037366103833No Hit
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG511520.1799589252337369No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT503590.17716905528319044Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG497020.17485764978822319Illumina Single End PCR Primer 1 (100% over 32bp)
GTCCGAGCCGCGACGGGCGAGGGGCGGACGTTCGTGGCGAACGGGACCGT486240.1710651153535585No Hit
GGGGGGTTTGCGCGAGCGTCGGCTCCGCCTGGGCCCTTGCGGTGCTCCTG485890.17094198111866676No Hit
GAAAGAGAGAGCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTC478450.1683244990969687No Hit
AAAGAAGGGCGTGTCGTTGGTGTGCGCGTGTCGTGGGGCCGGCGGGCGGC476690.16770530980151324No Hit
CGGGGTTCGTCGGCCCCGGCCGGGTGGAAGGTCCCGTGCCCGTCGTCGTC473210.16648100369458996No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC455090.16010616844819625No Hit
GCCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGT421450.14827120941460437No Hit
GGGGGAGCCGCGGGGATCGCCGAGGGCCGGTCGGCCGCCCCGGGTGCCGC414590.14585777841072683No Hit
GTGGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTG398950.14035543717156582No Hit
GGGGGGGCCTGGTGGGGTTGCGCGCACGCGCGCACCGGCCGGGCCCCCGC382640.1346173818256121No Hit
CGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGGGGCGTGTTG374410.13172196824515842No Hit
GCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCGGGGGG368140.12951610638009836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG363990.12805608616638237No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG362430.127507259290865Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG361590.1272117371271249Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGG358250.1260366847141583Illumina Single End PCR Primer 1 (97% over 35bp)
CCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGGGGCGTGTT353260.12428114233670219No Hit
CGTTGGTGTGCGCGTGTCGTGGGGCCGGCGGGCGGCGGGGAGCGGTCCCC353110.12422837052174861No Hit
CCGAGGGCCGTCCGGCGTCCCAGGCGGGGCGCCGCGGGACCGCCCTCGTG352990.12418615306978573No Hit
GTCGGGTTCGTGGCTGCGGTCGCTCCGGGGCCCCCGGTGGCGGGGCCCCG348940.12276131406603878No Hit
CTTCGGAGGCGCTCTGCCTCGGAAGGAAGGAGGTGGGTGGACGGGGGGGC347670.12231451269943173No Hit
CGCGAGCGTCGGCTCCGCCTGGGCCCTTGCGGTGCTCCTGGAGCGCTCCG340950.11995033538951087No Hit
GGGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAG336620.11842698899785058No Hit
CGGTGCCCTCCTCCCCGCCGGGGCCCGTCGTCCGGCCCCGCCGCGCCGGC326940.11502144787284554No Hit
GGAGGAGGAAGGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGG326420.11483850558100643No Hit
GGGGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCG326330.11480684249203428No Hit
GCCCGTCGTCGTCGTCGTCGCGCGTCGTCGGCGGTGGGGGCGTGTTGCGT324440.11414191762361904No Hit
GGCGGGTCCGGAAGGGGAAGGGTGCCGGCGGGGAGAGAGGGTCGGGGGAG322400.1134242209402502No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG322260.11337496724629352Illumina Single End PCR Primer 1 (100% over 30bp)
GTTCGAGGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGG321270.11302667326759983No Hit
GAGAGAGCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGG310000.10906175090408674No Hit
GGGGGACCGGCCGCGACTGCGGCGGCGGCGGTGGTGGGGGGAGCCGCGGG309890.10902305157312077No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA309790.1089878703631517No Hit
GTCGGCCCCGGCCGGGTGGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGC308780.10863254014246419No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG306870.10796057903205514No Hit
GGGCGCCCGCCTGCTCTCGGTGAGCGCACGTCCCGTGCTCCCCTCTGGCG306010.10765802062632124No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT302150.10630002592151551No Hit
GCCGGGTGGAAGGTCCCGTGCCCGTCGTCGTCGTCGTCGCGCGTCGTCGG296010.1041398996294152No Hit
CGGGGGTCGGCCTGCGGCGCGTGCGGGGGAGGAGACGGTTCCGGGGGACC295200.10385493182866583No Hit
CGCGAGGCGGTTCTCGGTGGGGGCCGAGGGCCGTCCGGCGTCCCAGGCGG292520.102912075401495No Hit
CCTCGCGCCGCGCGCGGCTCGGGGTTCGGGGTTCGTCGGCCCCGGCCGGG284250.10000258933705372No Hit
GGCGTGTTGCGTGCGGTGTGGTGGTGGGGGAGGAGGAAGGCGGGTCCGGA284250.10000258933705372No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)