FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23709786
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6347172.677025427391036Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC6303072.658425512571054Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC5783242.439178489422047Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG5665272.3894226628616555Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTAT4382941.8485784730406254TruSeq Adapter, Index 6 (97% over 38bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG4323231.8233947788478564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG4312601.8189113980193663TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC3965991.6727228158027236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC3790991.5989136300091447Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA3359261.4168242598225054Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT3264411.3768196811223856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC3233401.3637406933997631Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA3233001.363571986689378Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG3221101.3585529620554144Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT2995561.2634276834046498Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCA2784391.174363193324478TruSeq Adapter, Index 6 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG2755491.1621741334991382Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT2432871.0261037362378556No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG2391721.0087480334069654No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC2323590.9800130629605851No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC2161880.9118091576195584No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCGCGTAT1908430.8049123682516578TruSeq Adapter, Index 6 (97% over 38bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA1685840.7110313015899848No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAG1632190.6884035140595532TruSeq Adapter, Index 6 (97% over 38bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG1571140.6626546523869933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1564930.6600354807082611No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG1539270.6492129452370426TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG1491750.6291705880432662No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA1055360.4451157846806377No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCA1015590.428342120000577TruSeq Adapter, Index 6 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1006390.42446186566171457No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA965150.4070682038209877No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC754610.31826942680967263No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA739790.31201884318989637No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA729750.30778430475922475No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTT723770.30526213943896413TruSeq Adapter, Index 6 (97% over 38bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT628870.2652364724000461Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA487700.20569565663730577No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA444360.18741628456705597No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT392090.16537053518745382No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA372180.15697315867802433No Hit
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAG359730.15172216231728114TruSeq Adapter, Index 6 (97% over 38bp)
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG357290.15069305138393066Clontech Universal Primer Mix Short (95% over 21bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG347800.14669048468003887No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA316210.13336687222735794No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA306030.12907328644805144No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT298770.1260112596545578No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA278380.11741143509266597No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG276430.1165889898795375Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT274830.1159141630379962No Hit
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC266140.11224900975487506Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCA259260.10934725433624749No Hit
GGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAAAATCGAAGA257130.10844889110344565No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG239930.10119450255687673No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)