FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23709786
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC5850512.467550740441099Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5838522.462493756797299Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG5385632.271479801631276Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG5264872.2205472457659465Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG3977031.6773791210093587Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT3771001.5904825121576383Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG3652681.5405790672256594Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC3499931.476154192197264Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG2953201.2455616427748442Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA2939981.239985885996609Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG2926011.2340938041364018Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG2705631.1411448420496078Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG2704791.1407905579577986Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA2617751.1040799777779522Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG2140600.9028339606270592Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT1852340.7812554697878757No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCT1831500.7724658501768005Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT1566150.6605500361749364No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1407700.59372109052355No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT1337750.5642185045449166Illumina Single End PCR Primer 1 (96% over 33bp)
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1196280.5045511587493873No Hit
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT1178600.497094322150356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1126230.4750063960931574No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG1042620.4397424759548652No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT991660.418249241051775No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA984560.4152546969424355No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCTGA974620.4110623351893602Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC827350.34894874209324367No Hit
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC787620.3321919480842214No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCT675730.2850004635216868Illumina Single End PCR Primer 1 (96% over 33bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG669750.2824782982014262No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC624830.26353253462515436No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG613740.2588551410797213No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA529890.2234899969151978No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT496590.20944516327561963Illumina Single End PCR Primer 1 (100% over 31bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG437970.18472119486865043No Hit
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCTGAGG423690.17869836530789437Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCG371740.15678758129660048No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA369980.15604527177090505No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC369610.15588921806379863No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT335290.1414141823127379No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTGT310200.13083205390381844Illumina Single End PCR Primer 1 (96% over 33bp)
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA301090.1269897585747927No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG297320.12539969782941102No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT277900.11720898704020356No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG251960.10626835687171532Illumina Single End PCR Primer 1 (100% over 30bp)
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG242200.10215191313831344Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)