FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4296456
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1209462.8150177727876184Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1187442.763766229655325Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1102312.565626181206092Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1078582.510394613607122Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCTCGTAT885662.0613733737759676TruSeq Adapter, Index 6 (97% over 38bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG815371.8977734206983616TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG813141.8925830963938652Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC755381.75814671440834Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC717671.6703767011695219Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA627671.4609017292391682Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT615361.4322502080784723Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC607661.4143284604799864Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG591841.3775074154140063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA588251.3691516915336732Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT552211.2852686027740072Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT549451.2788447036348098No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCA529481.2323645348631524TruSeq Adapter, Index 6 (97% over 37bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG501011.1661006187425171Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC422650.9837177431818225No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG399350.9294870004487419No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTTATCGCGTAT389120.905676678639325TruSeq Adapter, Index 6 (97% over 38bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC387740.9024647290697263No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA339590.7903956190869871No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAG311980.7261333526981307TruSeq Adapter, Index 6 (97% over 38bp)
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG295160.6869848079440357TruSeq Adapter, Index 2 (97% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG277220.6452294635392519No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG257310.5988889447488814No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256360.5966778200451721No Hit
CTATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAGAGATCGGAAGAGCA247650.5764052977616901No Hit
TATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCA196320.456934738770745TruSeq Adapter, Index 6 (97% over 37bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA182620.42504799304356894No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163950.3815935738664611No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA161540.37598429961810387No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC138950.32340608166358503No Hit
ATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAGTT137410.31982173214388787TruSeq Adapter, Index 6 (97% over 38bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT122420.28493251181904344Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA120610.2807197373835552No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCA79110.18412850032678096No Hit
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA78620.18298802547960458No Hit
TATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCAAG70000.16292497816805293TruSeq Adapter, Index 6 (97% over 38bp)
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA63550.1479126051797109No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT62380.1451894305446163No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG59130.13762505655824242Clontech Universal Primer Mix Short (95% over 21bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56060.13047963251572925No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG55150.12836160779954456No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA53600.12475398328296625No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT53230.12389280839836367No Hit
ATATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAG50840.11833008414376874Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA49080.11423368469268624No Hit
CGGGAATCCGGCCGGCCCCGAAGACGGGGAGCCGGCGCGGCGGGGCCGGA48640.11320958482991562No Hit
ATATATATATATATATGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC48080.11190618500457122Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATCCCCTGTGTGCCTTGGCAGTCTCAGGGGATAAGATCGGAAGAGCACA47340.11018383523536607No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT47280.11004418525407918No Hit
ACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCA46230.10760031058155838No Hit
GACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCC44110.10266601124275448No Hit
GGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAAAATCGAAGA43790.10192121134255769No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)