FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4296456
Sequences flagged as poor quality0
Sequence length151
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC1084972.525267336614177Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1066052.48123104251504Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG992752.3106253153762077Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG966042.2484577987066547Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG718121.6714240760291736Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT690521.6071850846371984Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG667221.552954341904118Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC645261.501842448753112Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA527801.2284543353871191Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG524551.2208899614007451Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG509861.1866989909823351Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA453031.0544271837067574Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG445591.0371105860271814Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG443621.032525411641595Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCT360220.8384119376528004Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG331310.7711239216693945Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT294860.6862865580376012No Hit
CTATCCCCTGAGACTGCCAAGGCACACAGGGGATAGAGATCGGAAGAGCG292610.6810496837393424No Hit
ATACGACTCACTATAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGA281220.654539462291712No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTT248660.5787560724466863Illumina Single End PCR Primer 1 (96% over 33bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT234920.5467762267319857No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222060.5168445807428262No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG207070.4819553604179817No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCTGA186730.4346140167617218Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGT183240.42649104285020023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA175710.408964970198694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG173120.40293674600647605No Hit
TTGTACTCTAGTTGTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC160320.3731447499986035No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGT140920.3279912560491717No Hit
TATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCT131370.30576363402767304Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCGTC122370.28481613683463763No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCG95610.22253224518067913No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT92050.2142463462909896Illumina Single End PCR Primer 1 (100% over 31bp)
ATGTACTAGGAGCATAGGCTACTAGTGGCTGTGATCCAAGTATGAGTGTA89590.2085206970582266No Hit
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCGTC87170.20288814781298817No Hit
ATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCTGAGG80010.1862232500460845Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG74280.17288667683318532No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA62330.14507305556021055No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC61530.14321105580971852No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT56590.1317132073504302No Hit
ATATATATATATATATATATATATATATATATATATAGATCGGAAGAGCG55700.12964173262800782No Hit
TATCCCCTGAGACTGCCAAGGCACACAGGGGATAAGATCGGAAGAGCGTC51540.11995933392544925No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA49720.11572328449307988No Hit
ACATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG45870.10676241069383698Illumina Single End PCR Primer 1 (100% over 30bp)
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT45840.10669258570319352No Hit
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG44180.10282893622092254No Hit
ACATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG43450.10112986144859856Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)