FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26911264
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC359560.13360948040196105Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC309300.11493328592815262Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT276410.10271163777368465No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)