FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992751
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC61590.15425454780425826Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC52050.13036124717018416Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT42480.10639281037059412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)