FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3992751
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG72420.1813787035555185No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG58350.146139841928535Illumina Single End PCR Primer 1 (100% over 32bp)
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC56740.14210753437917867No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTC50840.1273307551610406Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT45220.11325524682105144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)