FastQCFastQC Report
Thu 12 May 2022
EGAF00005408408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46453932
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1889920.40683746641726687No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1416620.3049515808478817Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1410140.30355665048977987No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1365810.2940138630245552No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1345040.2895427667995898Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1243500.2676845525153823No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG1115090.24004211311972473Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1093850.23546984139039082Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA1063580.22895370837499826No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1030390.22180899563033757No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC1016090.21873067709316835No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG998420.2149269086629739Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC936810.20166430690947754No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA891540.19191916843551585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC887310.19100858889619937TruSeq Adapter, Index 5 (97% over 35bp)
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT860100.18515117299435493No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC850540.1830932201820935Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT840160.18085874840476368No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC835590.17987497807505295Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT834960.17973935984579303No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA822300.17701407923876067No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG819810.17647806433263818Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT818220.17613578975402985Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG812150.17482911887846223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA792790.1706615491666023Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC772780.16635405588487107No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT772710.16633898719273107TruSeq Adapter, Index 2 (97% over 36bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT761060.1638311262865757Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA755900.1627203484088279No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC749740.16139430350050885No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG725470.15616977266854395No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG719230.1548265063977792No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA714250.15375447658553426No Hit
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC692150.14899707520990904Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT662840.14268759854386492No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG661440.14238622470106516No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC639250.1376094492926885No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG637130.1371530831878774No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC635600.13682372463110334No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG633320.13633291580140083No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC631860.13601862593676678No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG609960.13130427796725583No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAA600680.12930659992355437TruSeq Adapter, Index 2 (97% over 36bp)
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT598470.12883085978599185No Hit
GCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCC592410.12752634158072992No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC590470.12710872354142164No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC586520.1262584187706651No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC582760.1254490147357171No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT552890.11901898853255306No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG525080.11303241241236586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTAT500060.10764643130747253TruSeq Adapter, Index 2 (97% over 36bp)
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG500030.10763997329655539No Hit
CAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCCT471140.10142090878335122No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)