FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005408409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46453932
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG2624790.5650307491731809No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC1934350.4164017805855487No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA1564570.3368003380209021No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1523760.3280152905032883No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1475340.3175920608830271Illumina Single End PCR Primer 1 (100% over 32bp)
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA1424200.306583304939612No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG1406420.3027558571360547Illumina Single End PCR Primer 1 (96% over 33bp)
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1384600.29805873052898946No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1262360.2717444887119566No Hit
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1160570.24983245767010637Illumina Single End PCR Primer 1 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG1137970.24496742277919553Illumina Single End PCR Primer 1 (100% over 32bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT1117890.2406448608053243No Hit
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG1036890.22320823132905088Illumina Single End PCR Primer 1 (100% over 30bp)
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG1011220.21768232665428625No Hit
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC1005610.21647467861278138No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA943180.20303555789421657No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC928170.1998043997653417No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCT927560.19967308687669325Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG878030.1890109108524979Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG864060.18600363043541718Illumina Single End PCR Primer 1 (100% over 24bp)
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA858090.18471848626290666No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG853930.18382297541573014Illumina Single End PCR Primer 1 (100% over 28bp)
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT846690.1822644421143941No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA840790.18099436663402357No Hit
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGG834660.17967477973662166No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA806500.1736128601557345Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG800390.172297578598944Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG798800.17195530402033565Illumina Single End PCR Primer 1 (100% over 24bp)
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC771700.16612156749185408No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA769830.1657190181446858Illumina Single End PCR Primer 1 (100% over 26bp)
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC765110.16470295776038937No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG746640.1607269757057379Illumina Single End PCR Primer 1 (100% over 22bp)
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG725900.1562623374916896No Hit
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG721360.15528502517289602Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG700950.15089142507893627No Hit
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG688200.1481467704391525No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC686000.14767318297189566No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA679790.14633637471204805No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG673510.14498449776006045No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC643710.13856954024903642No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAT620390.13354951309611424Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT597600.12864357746939484No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG589070.12680734969862184No Hit
GCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGA582250.12533922855012575No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT549110.11820527915699364No Hit
CTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCG549060.11819451580546507No Hit
GGGGGGTTTGCGCGAGCGTCGGCTCCGCCTGGGCCCTTGCGGTGCTCCTG518240.11155998592325835No Hit
GTTCGAGGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGG475990.1024649538816219No Hit
CCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCC474590.10216358003882212No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA464830.10006257382044646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)