FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755669
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT111470.40451157232599416No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC91830.3332403129693733Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC86790.3149507433585093Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG82850.3006529448928736No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG79360.2879881437139221No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA72470.262985140813356No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG71640.2599731680401383Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC71090.2579772824675242Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG65120.23631285179751266Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA64110.23264768010962128No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA60420.21925710235881016No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC60200.21845874812976449No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC56420.2047415709216165TruSeq Adapter, Index 5 (97% over 35bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC56220.2040157943497568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA55280.2006046444620163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCTCGTAT55090.1999151567187496TruSeq Adapter, Index 2 (97% over 36bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC55070.1998425790615636No Hit
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC54150.1965040068310091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT52850.19178645911392117Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT51450.1867060231109034No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA51310.1861979795106016No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG51120.18550849176733492Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG50910.18474642636688224Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA50830.18445611573813836Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT49720.1804280557643171No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT49620.18006516747838727No Hit
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT49310.17894021379200478Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC45180.16395292758310231Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC45180.16395292758310231No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC44850.16275539623953383No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA43550.1580378485224459No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG42860.15553391934953No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG42390.15382834440565976No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA42170.15302999017661412No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC40160.1457359356294243No Hit
GTCACACTCCCCCCACCCGCCAGCCGCTCCTCCTCGGGCCCGCAGAGGCG39600.14370376122821718No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC39140.14203447511293993No Hit
CCCGAACCCACCGCGATCGCTCACACGGCCCGCGCGCACCCGCCAGAGGG39000.14152643151263813No Hit
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAA38920.14123612088389426TruSeq Adapter, Index 2 (97% over 36bp)
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT37050.13445010993700623No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG36600.13281711265032192No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC36580.13274453499313596No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG35520.12889791916227963No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC35490.1287890526765007No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC35360.1283172979047919No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC35230.1278455431330831No Hit
GCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCC35150.12755523250433923No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT34420.1249061480170514No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT32420.11764838229845456No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAAGGTTATCGCGTAT31790.11536218609709656TruSeq Adapter, Index 2 (97% over 36bp)
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG31480.11423723241071407No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG30830.11187845855217009No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)