FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755669
Sequences flagged as poor quality0
Sequence length151
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG153500.5570335189023065No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC111990.4063985914128293No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG94990.34470758280475633Illumina Single End PCR Primer 1 (100% over 32bp)
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA91190.3309178279394223No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT90460.32826874345213447No Hit
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG89560.3250027488787659Illumina Single End PCR Primer 1 (96% over 33bp)
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA82390.2989836587775963No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT82140.2980764380627717No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC73110.26530762584330697No Hit
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG72730.26392865035677365Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG72220.2620779200985314Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG65660.23827244854153382Illumina Single End PCR Primer 1 (100% over 30bp)
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT65550.23787327142701103No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG62160.2255713585339894No Hit
GCCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTC59500.2159185301282556No Hit
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCT58050.2106566499822729Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG56120.20365290606382697Illumina Single End PCR Primer 1 (100% over 28bp)
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA55750.20231021940588656No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC54560.19799184880332144No Hit
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG53250.19323801225764053Illumina Single End PCR Primer 1 (100% over 28bp)
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA51390.18648829013934548No Hit
CCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGG50300.1825328078227102No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG49720.1804280557643171Illumina Single End PCR Primer 1 (100% over 24bp)
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT49550.1798111456782364No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA49530.17973856802105043Illumina Single End PCR Primer 1 (100% over 26bp)
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA49100.1781781483915521No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG47850.1736420448174291Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA47600.1727348241026045Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG45820.16627541261305331Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTG45750.16602139081290243Illumina Single End PCR Primer 1 (96% over 33bp)
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC45110.16369890578295143No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC44240.16054177769536182No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG43210.15680402835028445No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG41360.1500905950605824Illumina Single End PCR Primer 1 (100% over 22bp)
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA40370.14649800102987695No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG40190.14584480211520323No Hit
CGAGACGAGACGCGCCCCTCCCACGCGGGGAAGGGCGCCCGCCTGCTCTC39870.14468355960022775No Hit
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAT39440.14312313997072942Illumina Single End PCR Primer 1 (96% over 33bp)
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG38530.13982085656876786No Hit
GGCGGTTTGAGTGAGACGAGACGAGACGCGCCCCTCCCACGCGGGGAAGG38420.13942167945424505No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC38080.13818785928208357No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG34140.12389006081644785No Hit
GCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGA34110.12378119433066889No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT33630.12203933055820566No Hit
CTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCCCTCG31720.11510816429694568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30600.11104381549453145No Hit
GGGGGGTTTGCGCGAGCGTCGGCTCCGCCTGGGCCCTTGCGGTGCTCCTG30450.1104994830656367No Hit
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT29320.10639884543462948No Hit
CCGGCTCGGGCCGAGTTCCCGTGGCCGCCGCCTGCGGTCCGGGTTCCTCC28860.10472955931935223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)