FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3986116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG75460.18930708489165896Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA67020.16813359169677952Illumina Single End PCR Primer 1 (100% over 34bp)
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG63440.15915241804302735No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC50360.12633852100641327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)