FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005408424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7725544
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC168190.21770635181160058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC161570.20913737595695528Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA88270.11425732608603355TruSeq Adapter, Index 7 (100% over 35bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC79560.1029830391231996Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)