FastQCFastQC Report
Thu 12 May 2022
EGAF00005408434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32164222
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG469890.1460908956541837No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT447450.13911419962217647No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC442240.1374943873972764Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC438470.13632227759154256Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC404180.12566136373514644No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC358610.11149344759528149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)