FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64136571
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC4369570.6812914896869058Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4004750.6244097458842943Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA2400840.3743324537883386TruSeq Adapter, Index 7 (100% over 35bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2303710.3591882079258649Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG2287380.3566420786667875Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG2262690.35279248090765564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC1820030.28377413566434667Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1814660.2829368598455318Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT1674370.26106322397560044Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC1644450.25639817881751115Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACG1595540.24877226442305433TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1567820.24445023729129517Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1477220.2303241312978831Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCAATATCTCGTAT1414840.2205980110785779TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT1351440.2107128552288834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC1338190.20864695120666804No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG1317890.20548183032734943Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA1298750.2024975734982776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC1032180.1609347029169988No Hit
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG945280.14738549087696004No Hit
ATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCA925410.14428741443006052TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGCAATATCGCGTAT826220.1288219789611141TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG745620.11625504581465698No Hit
TATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA684950.10679554415218113TruSeq Adapter, Index 7 (100% over 35bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA648410.10109832656940766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)