FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005408439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64136571
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG4275870.6666820401109376Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA3885610.6058337605856727Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATG2301670.3588701366650861Illumina Single End PCR Primer 1 (97% over 34bp)
TATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG2270330.3539836889627292Illumina Single End PCR Primer 1 (100% over 32bp)
TATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2213480.3451197913277902Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG2168690.33813625614627885Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG1682380.2623121214260114Illumina Single End PCR Primer 1 (100% over 28bp)
TATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAG1562720.2436550591393481Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGA1531620.23880603158531813Illumina Single End PCR Primer 1 (97% over 34bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG1522450.23737627008466045Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA1467560.22881797032772455Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA1433900.22356979452487413Illumina Single End PCR Primer 1 (100% over 34bp)
TATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAA1238700.19313474055231297Illumina Single End PCR Primer 1 (100% over 26bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAG1192410.18591732944375838Illumina Single End PCR Primer 1 (100% over 22bp)
TATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAGGG1166640.1818993410171554Illumina Single End PCR Primer 1 (100% over 24bp)
TATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGTAG1105710.17239930086065872Illumina Single End PCR Primer 1 (100% over 22bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGT933930.14561582969566614No Hit
ATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATGGAAG881980.13751592675573504Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG831380.12962651215014284No Hit
TATATATATATATATATATATATATATATAAGATCGGAAGAGCGTCGTGT815880.12720979423736264No Hit
TATATATATATATAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATG657020.10244077439063588Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)