FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005408442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005408442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44151610
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1135600.2572046636577919Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1023090.2317220142142042Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT970950.21991270533509424No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG845970.1916056968250988No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC619610.14033689824674572No Hit
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG585550.13262257027546673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG569000.1288741225971148No Hit
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG565170.1280066570618829TruSeq Adapter, Index 2 (97% over 35bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA556020.125934252454214No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA537530.1217464097005749Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC489380.1108408051257927Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC485200.10989406728316362Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG479240.10854417313434324Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC475790.10776277467571396No Hit
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT471650.10682509652535886Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA469900.10642873498837302No Hit
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG459540.10408227468941676Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA447520.10135983716109108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)